31P NMR STUDIES OF INJURIES IN ERYTHROCYTES TREATED WITH METHYLENE BLUE PHOTOCHEMICAL METHOD

Abstract

Abstract:

In order to understand injuries occurred in erythrocytes treated with the methylene blue (MB) photochemical method, the changes of high-energy phosph a temetabolites in the treated erythrocytes were studied by ³¹P NMR. T he control and treated erythrocyte samples, which originally allcame from stored blood of healthy adults, were measured by ³¹P NMR with an MSL-300 MHz spectr ometer. L-hist idine was added to half of the treated samples before the treatment. The effects of the MB photochemical method on the erythrocytes were studied by measuring the relative changes of 2,3-DPG, ATP and (PME+SP) contents. In erythrocytes treat e d with MB photochemistry, the contents of (PME+SP) increased significantly, and the contents of 2,3-DPG and ATP decreased significantly. Our data suggest that, in the MB treated erythrocytes, glucose metabolism through the pentose phosphate pathway increases significantly. It is concluded that MB treatment may affect physical, chemical and functional properties of erythrocytes to some degree, and such effects can be ameliorated by L-histidine.

Key words: ³¹P NMR, erythrocytes, methylene blue photochemical method, L-hist idine

CLC Number:

O482.53

WU Chang-lin1, LAN Rong2, LU Ping3, HUANG Yong-ren1. ³¹P NMR STUDIES OF INJURIES IN ERYTHROCYTES TREATED WITH METHYLENE BLUE PHOTOCHEMICAL METHOD[J]. Chinese Journal of Magnetic Resonance.

References

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³¹P NMR STUDIES OF INJURIES IN ERYTHROCYTES TREATED WITH METHYLENE BLUE PHOTOCHEMICAL METHOD

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