DEVELOPMENT OF L-BAND THREE DIMENSIONAL ESR IMAGING SYSTEM（Ⅱ）——L-Band Three Dimensional ESR Imaging System

Abstract

Abstract:

An apparatus for ESR and ESR imaging at L-band (1.05 GHz) was built in our laboratory, consisting of L-band ESR spectrometer, three sets of gradient coils and a personal computer-based data acquisition system. The sample cavity is a 3-loop 2-gap reentrant resonator, accepting samples with sizes up to 20 mm in diameter and 30 mm in length. The resonance frequency of unloaded resonator is 1.05 GHz. The microwave oscillator frequency is locked to the resonant frequency of loaded resonator by means of automatic frequency control (AFC). Linear magnetic field gradients (up to 2 mT/cm in amplitudes) in the X-, Y-, and Z-directions are produced by gradient coils, and have a linear range of 40 mm measuring from the center of the magnet. All projections in imaging were acquired as single scans with constant sweep time. To accurately compute the spatial distribution of the spins, the line shape function that causes line-broadening of the ESR signals needs to be removed from the observed projections. Three- dimensional ESR image is constructed based on the Lauterbur's method. Using this system, we studied the spatial distribution of TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) nitroxide radicals in sample tube. Stack images of ESR-CT and 2D, 3D ESR images were obtained.

Key words: ESR, imaging system, L-band, field gradient, 3D image construction, ESR-CT

CLC Number:

O482.53

ZHENG Ying-guang, XU JING, DONG Feng-xia, SHEN Er-zhong, XU Yu-shu, WU Ke, CONG Jian-bo, XIAN Hong, WANG Chang-zheng, ZHANG Qing-jun. DEVELOPMENT OF L-BAND THREE DIMENSIONAL ESR IMAGING SYSTEM（Ⅱ）——L-Band Three Dimensional ESR Imaging System[J]. Chinese Journal of Magnetic Resonance.

References

［1］Lauterbur P C. Image formation by induced local interactions: Examples employing nuclear magnetic resonance［J］. Nature，1973，242: 190-191.

［2］Hoch M J R, Day A R. Imaging of Paramagnetic Centres In Diamond［J］. Solid State Commun，1979，30: 211-213.

［3］Keiichi Ohno. Two-Dimensional ESR Imaging For Paramagnetic Species With Anisotropic Parameters［J］. J Magn Reson，1985，64: 109-114.

［4］Berliner L, Wan X, Fujii H. Non-Invasive Visualization of Solvent Swelling and Diffusion into Solid Polymers by Electron Spin Resonance (ESR) Imaging［J］. Journal of Polymer Science: Polymer Letters Edition，1986，24: 587-595.

［5］Kuppusamy P, Wang P, Zweier J L. Three-Dimensional Spatial EPR Imaging of the Rat Heart［J］. J Magn Reson Med，1995，34: 99-105.

［6］Hitoshi Togashi，Taku Matsuo，Haruhide Shinzawa, et al. Ex vivo measurement of tissue distribution of a nitroxide radical after intravenous injection and its in vivo Imaging using a rapid scan ESR-CT system［J］. Magn Reson Imaging，2000, 18: 151-156.

［7］Mobae Afeworki，Gooitzen M van Dam, Nallathamby Devasahayam，et al. Three-Dimensional Whole Body Imaging of Spin Probes in Mice by Time-Domain Radiofrequency Electron Paramagnetic Resonance［J］. Magn Reson Med，2000, 43: 375-382.

［8］郑莹光，徐玉书，裘祖文. ESR成像——TEMPOL水溶液样品的X波段一维成像［J］. 高等学校化学学报，1987，12：1118-1120.

［9］郑莹光，沈尔中. 石英玻璃中顺磁缺陷中心的ESR成像［J］. 高等学校化学学报，1992，13（7）：981-984.

［10］梁明理，周建平, 刘军, 等. 测试生物样品的ESR成像系统［J］. 波谱学杂志，1990，7（3）：351-357.

［11］Xu Yuanzhi，Furusawa M，Ikeya M, et al. ESR microscopic imaging on the spatial distribution of paramagnetic reactive centers on catalysis［J］. Chem Lett，1991，230(2): 293-296.

［12］何光龙，傅乐虞，徐广智. X-波段电子自旋成象［J］. 波谱学杂志, 1996，13（2）：191-198.

［13］何光龙，傅乐虞，徐广智. Al₂O₃-SiO₂石油裂化催化剂上自由基空间分布的ESR成象［J］. 波谱学杂志. 1996，13（4）：383.

［14］吴可，丛建波，先宏, 等. L波段三维ESR成像系统的研制（Ⅰ）［J］. 波谱学杂志，2002，19（4）：337-343.

［15］Shin -Ichi Ishida, Seiji Matsumoto, Hidekatsu Yokoyama, et al. An ESR-CT Imaging of The Head of a Iiving Rat Receiving an Administration of a Nitroxide Radical［J］. Magn Reson Imaging，1992, 10（1）: 109-114.

［16］郑莹光，沈尔中, 王萍, 等. 卷积差技术在ESR和ESR成像中的应用［J］. 波谱学杂志, 1992，9（1）：99-103.

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