Abstract: Following the 50-year brilliant evolution of NMR techniques,the outlines of muitidimensional NMR,especially 2D NMR-the core of modern NMR spectrosocpy,are given in this nunireview article.The text falls into three sections.After a brief introduction of the Pulsc Fourier Tramform NMR,the fundainental mechanisms and experimental categories,as well as the extensive applications of 2D NMR are introduced in some detail in the next section.The insights into the design and the usage of the most useful 2D NMR pulse sequences,including COSY types,NOESY (ROESY),heteronuclear chemical shift correlation experiments are described with application examples adopted from our laboratory.Multiple quantum coherences,such as INADEQUATE and HMBC/HMQC are mentioned as well.Finally,foundational experimental methods and categories and enchanting perspectives of 3D/4D NMR in the fields of chemistry,biology and medicine are summarized in the third section.

Key words: Pulse Fourier tranform NMR, 2D NMR, 3D and 4D NMR