Abstract: A new NMR technique for measurement of 1D Homonuclear Total Correlation,called the one-dimensional spin echo MDY coherence transfer (1D-SEMDY) was developed.The results obtained show that the efficiency of magnetization transfer among sugar residues of glycoside are much batter for this method than that for 1D-HOHAHA,1D-relayed HOHAHA or 1D multiple relay COSY and a 1D-TOCSY spectrum with all peaks in the absorption mode can be obtained without the use of the Z-filter.The subspectra obtained by the technique show clear lineshapes and good resolution.Spectral assignment within the subspectra can be achieved by varation of mixing time and delay time.

Key words: 1D-SEMDY NMR techniques, Structure analysis of glycoside, NMR subspectra, Mixing pulse