Chinese Journal of Magnetic Resonance ›› 1985, Vol. 2 ›› Issue (4): 297-302.

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THE HIGH RESOLUTION NMR STUDIES OF HISTONE,PRO-TAMINES AND THEIR INTERACTION WITH DNA

Yuan Chuanzhao1, Wu Jun1, Zeng Weiqiang1, Dai Peilin2, Xia Pong2, Zhang Chenfang2   

  1. 1. Institute of Genetics, Acaderaia Sinica;
    2. Wuhan lastutite of Physics, Academia Sinica
  • Received:1984-09-18 Revised:1985-07-12 Published:1985-12-05 Online:2018-01-23

Abstract: In this paper we present some results of high resolution NMR studies of hi-stones, protamines and their interaction with DNA.
Differences were found in the spectral characteristics of histones and protamines.
The spectra of protamines showed that the Arg-δCH2 peak was rather strong while the Lys-εCH2 peak was rather weak. The intensity of proton resonances of Val, lie and Leu-CH3 in protamines was relatively low and weak.
When protamines interacted with DNA. the intensity of proton peaks of Arg-δCH2 became weak,but the Arg-δCH2 and Arg-βCH2 proton peaks of protamines did not show any obvious change.