Applications of Electron Spin Resonance in Biology and Medicine

Abstract

Abstract:

Electron spin resonance (ESR) is the most direct technique and an effective and unique way to detect free radicals in biological samples. This paper reviews the applications of ESR in biology and medicine. The topics reviewed include development of spin labeling and spin trapping, ESR imaging and applications of ESR in studies of cell membrane, protein structure, and diseases (i.e., heart diseases, Alzheimer's disease, Parkinson’s disease, stoke, radiation damage and plant diseases).

Key words: ESR, spin labeling, spin trapping, ESR imaging, biology, medicine

CLC Number:

O482.53

ZHAO Bao-Lu. Applications of Electron Spin Resonance in Biology and Medicine[J]. Chinese Journal of Magnetic Resonance, 2010, 27(1): 51-67.

References

[1] Xu Guang-zhi(徐广智). Basic principle of electron spin resonance spectroscopy(电子自旋共振波谱基本原理)
[M]. Beijing(北京): Science Press(科学出版社)，1982.

[2] Zhao Bao-lu(赵保路). Application of electron spin resonance technique on biology and medicine(电子自旋共振技术在生物和医学中的应用)[M]. Hefei(合肥): University of Science and Technology of China Press(中国科学技术大学出版社)，2009. 

[3] Lawrence J Berliner. Spin Labeling. Theory and application[M]. New York: Academic Press, 1976.

[4] Lawrence J Berliner. Spin Labeling. Theory and application[M]. New York: Academic Press, 1978.

[5] Lawrence J Berliner. Biological Magnetic Resonance Spin Labeling. The next Millenium[M]. New York: Academic Press, 1998. 

[6] Zhang Jian-zhong(张建中)，Zhao Bao-lu(赵保路)，Zhang Qing-gang(张清刚). Basic principle and application of electron spin resonance and spin labeling(电子自旋共振自旋标记的基本理论和应用)[M]. Beijing(北京): Science Press(科学出版社)， 1987.

[7] Zhao Bao-lu(赵保路), Zhang Qing-gang(张清刚), Zhang Jian-zhong(张建中), et al. Studies on the membrane fluidity of the lung cell of Chinese hamster with fatty acid spin labels(用脂肪酸自旋标记研究中国地鼠肺正常细胞V79和癌变细胞V79-B1 膜的流动性)[J]. Chinese Sci Bull(科学通报), 1982, 27: 686-689.

[8] Zhao Bao-lu(赵保路), Zhang Qing-gang(张清刚), Zhang Jian-zhong(张建中), et al. Studies on dependence of membrane fluidity of normal cell V79 and cancer cell V79-B1 of Chinese hamster lung with fatty acid spin labels on temperature(用脂肪酸自旋标记研究中国地鼠肺正常细胞V79和癌变细胞V79-B1膜的温度相关性)[J]. Chinese Sci Bull(科学通报), 1982, 27: 813-816.

[9] Xin Wen-juan(忻文娟), Zhao Bao-lu(赵保路), Zhang Jian-zhong(张建中). Study on binding sites of sulfhydryl groups on the membrane of normal cell V79 and cancer cell V79-B1 of Chinese hamster lung with maleimide spin labels(用马来西亚胺自旋标记研究中国地鼠肺正常细胞V79和癌变细胞V79-B1巯基结合位置的性质)[J]. Sci China Ser B(中国科学B辑), 1984, 26(5): 429-435.

[10] Xin Wen-juan(忻文娟), Zhao Bao-lu(赵保路), Zhang Jian-zhong(张建中). Study on dependence of membrane of binding sites of sulfhydryl groups on the membrane of normal cell V79 and cancer cell V79-B1 of Chinese hamster lung with maleimide spin labels(温度对中国地鼠肺正常细胞V79和癌变细胞V79-B1膜上巯基结合位置的影响)[J]. Chinese Sci Bull(科学通报), 1985, 30: 142-145.

[11] Shi H L, Yang F J, Zhao B L, et al. Effects of r-HuEPO on the biophysical characteristics of erythrocyte membrane in patients with anemia of chronic renal failure[J]. Cell Res, 1994, 4: 57-64.

[12] Zhao Bao-lu(赵保路), Xin Wen-juan(忻文娟), Zhaou Feng-lan(周凤兰), et al. Studies on the property of sulfhydryl groups binding sites on the red cell from PNH with spin labels(用自旋标记ESR波谱研究PNH患者红细胞膜蛋白巯基的性质)[J]. Acta Biochimica et Biophysica Sinica(生物化学与生物物理学报), 1985, 17: 175-180.

[13] Zhao B L, Beliner L. Spin-label and fluorescence labeling studies of the thioester bonds in human a2-M
[J]. Biochemistry, 1988, 27: 5 304-5 308.

[14] Zhao Bao-lu(赵保路). Oxygen free radicals and natural antioxidants(氧自由基和天然抗氧化剂)[M]. Beijing(北京): Science Press(科学出版社), 1998. 2002. 

[15] Zhao B L, Li X J, Xin W J. ESR study on active oxygen radicals-produced in the respiratory burst of human polymophonuclear leukocytes[J]. Cell Biol Int Rep, 1989, 13: 529.

[16] Zhao B L, Duan S J, Xin W J. Lymphocytes can produce respiratory burst and oxygen radicals as polymorphonuclear leukocytes[J]. Cell Biophys,1990, 17: 205-212.

[17] Zhao Bao-lu(赵保路), Huang Ning-na(黄宁娜), Zhang Jian-zhong(张建中), et al. Studies on the exposition of hydroxyl radical from hemotoporphyrin derivatives to light by spin trapping method(用自旋捕集技术研究光照血卟啉衍生物水溶液产生的羟基自由基)[J]. Chinese Sci Bull(科学通报), 1985，30: 1 743-1 746. 

[18] Zhang Jian-chao(张建朝)，Zhao Bao-lu(赵保路)，Xin Wen-juan(忻文娟). ESR study on the mechanism of lipid peroxidation in linolic acid(亚油酸体系脂质过氧化起动机理的ESR研究)[J]. Acta Biophysica Sinica(生物物理学报)， 1991, 7: 189-193.

[19] Zhang J C, Zhao B L, Xin W J. Evidence for L. against LOO. being spin-trapping by 4-POBN during the reaction of Fe-induced lipid peroxidation[J]. Appl Magn Reson, 991, 2: 521-530.

[20] Zhao W E, Han Zhao B L, Hou J W, et al. Effect of Carotenoids on the Respiratory Burst of Rat Peritoneal Macrophages[J]. Biochim Biophys Acta，1998, 11 381: 77-88.

[21] Zhao Bao-lu(赵保路)，Shen Jian-gang(沈剑刚)，Hou Jing-wu(侯京武)，et al. Studies on nitric oxide free radicals generated from myocardium from the injury of ischemia-reperfusion and polymorphonuclear leukocytes (PMN) by ESR(用ESR研究心肌缺血再灌注组织和多形核白细胞产生的NO自由基)[J]. Chinese J Magn Reson(波谱学杂志), 1997，14(2): 99-106. 

[22] Zhao Bao-lu(赵保路)，Wang Jian-chao(王建潮)，Hou Jing-wu(侯京武)，et al. The nitric oxide free radicals generated from polymorphonuclear leukocytes (PMN) mainly form ONOO^-(多形核白细胞产生的NO和O^-₂自由基主要形成ONOO^-)[J]. Sci China Ser C(中国科学C辑)，1996, 26(5): 406-413.

[23] Zhao Bao-lu(赵保路)，Shen Jian-gang(沈剑刚)，Hu Jun-gai(呼俊改). Synergic effect of nitric oxide and oxygen free radicals generated from myocardium from the injury of ischemia-reperfusion(NO和氧自由基在心肌缺血再灌注损伤中的协同作用——心肌缺血再灌注损伤产生的NO自由基的ESR研究)[J]. Sci China Ser C(中国科学C辑)，1996, 26(4): 331-338.

[24] Zhao B L, Shen J G, Li M, et al. Scavenging effect of Chinonin on NO and oxygen free radicals generated from ischemia reperfusion myocadium[J]. Biachem Biophys Acta, 1996, 1317: 131-137.

[25] Li H T, Zhao B L, Hou J W , et al. Two peak kinetic curve of chemiluninencence in phorbol stimulated macrophage[J]. Biochem Biophys Res Commn, 1996, 223: 311, 314.

[26] Zhao B L, Wang J C, Hou J W, et al. Studies on the mechanism of generation of NO free radicals from polymorphonuclear leukocytes stimulated by PMA[J]. Cell Biol Int Rep, 1996, 20: 343-350.

[27] Cao Y, Guo P, Zhao B L. Simultaneous detection of NO and ROS by ESR in biological system[J]. Method Enzymol, 2005, 396: 77-83.

[28] Zhao Bao-lu(赵保路). EPR imaging(顺磁成象)[J]. Progress in Biochemistry and Biophysics(生物化学与生物物理进展)，1990, 17: 277-289.

[29] Zhao Bao-lu(赵保路). Applications of In RES vitro and in Vivoand EPR imaging(体内EPR和EPR成像在生物和医学中的应用)[J]. Chinese J Magn Reson(波谱学杂志)，2000, 17(4): 343-348. 

[30] Wu Ke(吴可)，Cong Jian-bo(丛建波)，Xian Hong(先宏)，et al. L-band three dimensional ESR imaging system (Ⅰ)——Magnet and magnetic gradients for L-band 3D-ESRI(L波段三维ESR成像系统的研制（Ⅰ）——L波段ESR成像的磁场及三维梯度磁场系统)[J]. Chinese J Magn Reson(波谱学杂志), 2002, 19(4): 337-343. 

[31] Zhang Ying-guang(郑莹光), Xu jing(许静)，Dong Feng-xia(董凤霞)，et al. Development of L-band three dimensional ESR imaging system (Ⅱ)——L-band three dimensional ESR imaging system(L波段三维ESR成像系统的研制（Ⅱ）——L波段三维ESR成像系统)[J]. Chinese J Magn Reson(波谱学杂志), 2003, 20(2): 105-112.

[32] Zhang Ying-guang(郑莹光), Dong Feng-xia(董凤霞)，Xu jing(许静)，et al. Development of L-band three dimensional ESR imaging system (Ⅲ)——Development of L-band resonator(L波段三维ESR成像系统的研制（Ⅲ）——L波段谐振腔的研制)[J]. Chinese J Magn Reson(波谱波杂志), 2003，20(3): 231-237. 

[33] Wu Ke(吴可)，Cong Jian-bo(丛建波)，Zhong Qing-jun(张清俊)，et al. Development of L-band three dimensional ESR imaging system (Ⅳ)——System configuration and performance features(L波段三维ESR成像系统的研制（Ⅳ）——系统组成与各部分性能指标)[J]. Chinese J Magn Reson(波谱学杂志)，2004, 21(1): 33-40. 

[34] Wu Ke(吴可)，Cong Jian-bo(丛建波)，Zhong Qing-jun(张清俊)，et al. Development of L-band three dimensional ESR imaging system (Ⅴ)——System configuration and performance evaluation(L波段三维ESR成像系统的研制（Ⅴ）——灵敏度与分辨率实验及系统总性能指标)[J]. Chinese J Magn Reson(波谱学杂志)，2004, 21(2): 159-164. 

[35] Wu K, Huang C G, Cao Y L，et al. Plate form three-dimensional gradient coils for L-band ESR imaging experiment[J]. J Magn Reson, 2005, 175: 256-263；2007，184（2）：357.

[36] Zhao Bao-lu(赵保路). Study on L-band and X-band ESR imaging instruments(L-波段和X-波段ESR成像仪器的研制)[C]. Proceadings of 14^th National Magnetic Resonance Conference, Xian(第十四届全国波谱学学术会议，西安)，2006. 5-9. 

[37] Chen L J, Yang X Q, Jiao H L, et al. Tea catechins protect against Lead-induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 Cells[J]. Toxcol Sci, 2002, 69: 149-156.

[38] Altenbach C, Marti T, Khorana H G, et al. Transmemebrane protein structure:Spin labeling of bacteriorhodopsin mutants[J]. Science, 1990, 248(4959): 1 088-1 092. 

[39] Perozo E, Cortes D M, Sompornpisut P, et al. Open channel structure of MscL and the gating mechanism of mechanosensitive channels[J]. Nature, 2002, 418: 942-949.

[40] Nathalie Chomiki, John C Voss, Craig H Warden. Structure function relationships in UCP1, UCP2 and chimeras EPR analysis and retinoic acid activation of UCP2[J]. Eur J Biochem, 2001, 268: 903-913.

[41] Zhao Bao-lu(赵保路), Xin Wen-juan(忻文娟), Yang Wei-dong(杨卫东), et al. Study on the free radicals produced from ischemia-reperfusion heart by ESR spin trapping technique(用电子自旋共振直接检测兔心肌缺血再灌注产生的活性氧自由基)[J]. Chinese Sci Bull(科学通报), 1989, 34: 780.

[42] Yang Wei-dong(扬卫东), Zhu Hong-liang(朱鸿良), Zhao Bao-lu(赵保路), et al. Study on the free radicals produced from ischemia-reperfusion heart and scavenging effect of Danshen by ESR spin trapping technique(电子自旋共振直接检测心肌再灌注产生的氧自由基和复方丹参对氧自由基的清除作用)[J]. Chinese Journal of Cardiology(中华心血管病杂志), 1989, 17: 178.

[43] Zhao Bao-lu(赵保路). Free Radical, natural antioxidants and health(自由基和天然抗氧化剂和健康)[M]. Hongkong(香港): Chinese Scientific Culture Press(中国科学文化出版社), 2007.

[44] Zhao Bao-lu(赵保路). Nitric oxide free radicals(一氧化氮自由基)[M]. Beijing(北京): Science Press(科学出版社)， 2008.

[45] Zhao B L, Shen J G, Li M, et al. Scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on the myocardium from the injury of ischemia-reperfusion[J]. Biochem Biophys Acta, 1996, 1 315: 131-137. 

[46] Shen J G, Wang J, Zhao B L, et al. Effects of EGb761 on nitric oxide, oxygen free radicals, myocardial damage and arrhythmias in ischemia-reperfusion injury in vivo[J]. Biochem Biophys Acta, 1998，1 406: 228-236.

[47] Shen J G, Li M, Xin W J, et al. Effects of Chinonin on nitric oxide free radical, myocardial damage and arrhythmia in ischemia-reperfusion injury in vivo[J]. Appl Magn Reson, 2000, 19: 9-19.

[48] Nie G J, Jin C F, Cao Y L, et al. Distinct effects of tea catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells[J]. Arch Biochem Biophys, 2002, 397: 84-90.

[49] Nie G J, Cao Y L, Zhao B L. Protective effects of green tea polyphenols and their major component, epigallocatechin-3-gallate, on 6-hydroxyldopamine- induced apoptosis in PC12 cells[J]. Redox Report, 2002, 7: 170-177.

[50] Guo S H, Bezard E, Zhao B L. Protective effect of green tea polyphenols on the SH-SY5Y cells against 6OHDA induced apoptosis through ROS-NO pathway[J]. Free Rad Biol Med，2005, 39: 682-695.

[51] Guo S H, Yan J Q, Erwan Bezard, et al. Protective effects of green tea polyphenols in the 6-OHDA rat model of Parkinson’s disease through inhibition of ROS-NO pathway[J]. Biological Psychiatry, 2007, 62: 1 353-1 362.

[52] Zhang D L, Yin J J，Zhao B L. Oral administration of Crataegus extraction protects against ischemia/reperfusion brain damage in the Mongolian gerbils[J]. J Neur Chem, 2004, 90: 211-219.

[53] Xie Yu-xiang(谢渝湘)，Liu Qiang(刘强)，Zhang Jie(张杰)，et al. The mechanisms of prevent effect of nicotine on Parkinson's disease and Alzheimers' disease(尼古丁预防帕金森综合症和老年痴呆症的分子机理)[J]. Acta Tabacaria Sinica(中国烟草学报), 2006, 12: 25-30. 

[54] Xie Y X, Bezard Erwan, Zhao B L. Unraveling the receptor- independent neuroprotective mechanism in mitochondria[J]. J Biol Chem, 2005, 396: 84-92. 

[55] Liu Q, Tao Y, Zhao B L. ESR study on scavenging effect of nicotine on free radicals[J]. Appl Mag Reson, 2003, 24: 105-112.

[56] Zhang J, Liu Q, Liu N Q, et al. Nicotine reduces β-amyloidosis by regulating metal homeostasis[J]. FASEB J， 2006, 20: 1 212-1 214. 

[57] Liu Q, Zhang J, Zhu H, et al. Dissecting the Signalling Pathway of Nicotine-Mediated Neuroprotection in a Mouse Alzheimer Disease Model[J]. FASEB J，2007, 21: 61-73.

[58] Zhao Bao-lu(赵保路), Huang Ning-na(黄宁娜), Zhang Jian-zhong(张建中), et al. Studies on the expsition of hydroxyl radical from hemotoporphyrin derivatives to light by spin trapping method(用自旋捕集技术研究光照血卟啉衍生物水溶液产生的羟基自由基)[J]. Chinese Sci Bull(科学通报), 1985, 30:1 743-1 746.

[59] Zhang Jian-zhong(张建中), Huang Ning-na(黄宁娜), Zhao bao-lu(赵保路), et al. Studies on the expsition of hydroxyl radical from hemotoporphyrin derivatives by spin trapping DMPO(在血卟啉生物光敏体系中DMPO捕集自由基的ESR研究)[J]. Acta Chimica Sinica(化学学报), 1986, 44: 627-630.

[60] Zhao B L, Huang N N, Zhang J Z, et al. Studies on the expsition of hydroxyl radical from hemotoporphyrin derivatives to light by spin trapping method[J]. Chinese Sci Bull, 1986, 31: 1 139-1 143.

[61] Lu Z B, Tao Y, Zhou Z X, et al. Mitochondrial ROS and NO Mediated Cancer Cell Apoptosis in 2-BA-2-DMHB Photodynamic Therapy[J]. Free Rad Biol Med，2006, 41: 1 590-1 605. 

[62] Zhao Bao-lu(赵保路), Yan Liang-jun(晏良军), Hou Jing-wu(候京武), et al. ESR spin trapping studies on the free radicals in cigarette smoke(电子自旋共振自旋捕集吸烟气相自由基的研究)[J]. National Medical Journal of China(中华医学杂志)，1990, 70: 386-391.

[63] Yang F J, Zhao B L, Xin W J. Studies on toxicological mechanisms of gas-phase cigarette smoke and protective effects of GTP[J]. Res Chem Intermed，1992, 17: 39-57.

[64] Gao J T, Tang H R, Zhao B L. The toxicologic al damagement of gas phase cigarette smokeon cells and protective effect of green tea polyphenols[J]. Res Chem Intermed, 2001，27: 269-280. 

[65] Gao J T, Tang H R, Li Y, et al. EPR study of the toxicological effects of Gas-phase cigarette smoke and the protective effects of grape seed extract on the mitochondrialmembrane[J]. Appl Magn Reson, 2002，22: 497-551. 

[66] Xu Y C, Cao Y L, Guo P, et al. Detection of nitric oxide in plants by electron spin resonance[J]. hytopathology， 2004，94: 402-407.

[67] Guo P, Cao Y L, Li Z Q, et al. Role of an endogenous nitric oxide burst in the resistance of wheat to stripe rust
[J]. Plant Cell Environ，2004，27: 473-477. 

[68] Xu Y C, Cao Y L, Guo P, et al. Technique of detection of NO in plants by ESR spin trapping[J]. Method Enzym, 2005，396: 84-92. 

[69] Zhao B L, Li X J, Liu G T, et al. Scavenging effect of schisandrins on active oxygen radicals[J]. Cell Biol Int Rep, 1990,14: 99-105.

[70] Li X J, Zhao B L, Liu G T, et al. Scavenging effects on active oxygen radicals by schisandrins with different structures and configurations[J]. Free Rad Biol Med, 1990, 9: 99-108.

[71] Shi H L, Zhao B L, Xin W J. Scavenging Effects of Baicalin on Free Radicals and its Protection on Erythrocyte Membrane from Free Radical Injury[J]. Biochem Mol Biol Int, 1995, 35: 981-994.

[72] Shi H L, Yang FJ, Zhao B L, et al. Effects of r-HuEPO on the biophysical characteristics of erythrocyte membrane in patients with anemia of chronic renal failure[J]. Cell Res, 1994, 4: 57-64.

[73] Zhao B L, Jiang W, Zhao Y，et al. Scavenging effects of salvia miltiorrhza on free radicals and its protection fro myocardial mitochendrial membranrene from ischemia-reperfusion injury[J]. Biochem Mol Biol Int, 1996, 6: 1 171-1 182.

[74] Zhou G Y, Jiang W, Zhao Y, et al. Sodium tanshinone IIA sulfonate mediates electron transfer reaction in rat heart mitochondria[J]. Biochem Pharmacol, 2003, 65: 51-57.