Objective  The use of different models of focal cerebral ischemia to appraise the time-space rules of ¹H-MRS on measuring metabolism and energy changes in the post-ischemia brain tissue provides the clinical doctors with valuable informati on to judge the prognosis and carry out more effective therapy from the biochemi cal point of view. Methods 9 healthy Sprague-Dawly rats(indiscrimi nate the sex) was divided into two groups randomly. Group A (4 rats), occluded with self-thrombus for 1 h; Group B (5 rats), occluded with thread-emboli fo r 1 h. The ¹H-MRS was examined in 30, 40, 50,  60 min after occlusion  respectively, and the metabolic changes of NAA, C ho and Lac in the reg ions of interest in partly-fixed quantity were analyzed. Results Set the resonance intregrated area ratio of NAA, Cho, Lac to Pcr+Cr as the criterion, the values of a ll the metabolites declined gradually within 1 h after ischemia. Especially,  the ratio of Cho/（Pcr+Cr）、NAA/（Pcr+Cr）and Lac/（Pcr+Cr）in 60 min has  significant difference from that in 50 min (P<0.05). Conclusions Magnetic Reson ance Proton Spectroscopy is a good research tool, which is straightforward, comp rehe nsive and no damage, for the study of cellular metabolism and the status of the biochemical energy in acute ischemia stroke.

Photolysis of 2-methyl-naphthoquinone diazide produced 2-methyl-4-spiro (2′-tetrahydro-pyran)naphthalenone (3) and 2-methyl-4-(2′-tetrah ydrofur an)naphthol (4) in tetrhydrofuran, and 2-methyl-4-spiro［7′-(1 ′4′-dioxah eptane)］naphthalenone (5), 2-methyl-4-(2′-dioxanyl)naphthol (6) and bis(2 -methyl-1,4-naphthalene)22-crown-6 (7) in dioxane. The structur es of these  compounds were elucidated by 2D NMR spectroscopy and their ¹H and ¹³C NMR spectra were totally assigned. 

This paper reviews the main spectroscopic features(IR, UV, MS, ¹H NMR, ¹³C NMR,CD)spectra of dibenzylbutyrolactone lignans. All of these findings  will be useful for structural elucidation and assignments of the spectroscopic s ignals of such compounds.

Three protopanaxadiol bisdesmosides were elucidated as ginsenoside-Rd,gypenosi de XVII, and notoginsenoside-E on the basis of MS and NMR data comparison with  thos e in the references. The complete assignments of ¹H and ¹³C NMR  chemical  shifts of these ³ compounds were obtained by means of 2D NMR spe ctra,such as DQF ¹H-¹H COSY, HMQC, HMBC and TOCSY.

Like K⁺ channel, the pore region of HCN channel includes GYG characteristic se quence. But besides K⁺, Na⁺ can selectively overpass HCN channel. The conf ormation study of mHCN1 pore region peptide helps to understand the selectivity  of HCN channel. In this article, new developed WET sequence was used to suppress  solvent peak. With the comprehensive use of wet-TOCSY and wet-NOESY NMR exper iments, the ¹H resonance of mHCN1 pore region peptide was assigned.（95%H₂O+5%D₂O）.

We have measured the δ_Xe-Xe (arising from Xe-Xe collisions) by ¹²⁹Xe NMR spectroscopy,  which is much smaller than that of Xe in the gas phase and those of Xe adsorbed  in NaY and CaA zeolites. The Xe-Xe collisions in the supercages behaves like o n e dimensional gas collisions. This result suggusts that δ_Xe-Xe is al so related to the size and shape of internal cavity.

This Paper Reported the structure elucidation of three compounds, Neodiosbulbin  (1), Diosbulbin B (2) and 5-ureidohydautotion (3) isolated from Dioscorea bulbi fera L. On the basis of spectroscopic evidences, mainly modern 2D NMR techniqu e, their structures were determined. Neodiosbulbin (1) is a new fura nditerpenoid  compound, Diosbulbin B (2)  and 5-ureidohydautotion are  known fur anditerpenoid compounds.

Oleuropein is a well-known secoiridoid glucoside which is rich in the Oleaceae  family. Its structure was confirmed during 1960～1974. It has been isolated from  ma ny plants of the genus Olea, Fraxinus, Jasminum, Ligustrum and Syringa to d ate. Its  NMR data has been reported, but the detailed elucidation has not been given. Ou r recent study on the leaves of Syringa pubescens Turcz.(a  Chinese folk her b) has resulted in the isolation o f this constituent for the first time. In this paper, by utilizing a combination  of 1D and 2D NMR techniques, its ¹H and ¹³C NMR spectra are  completely assigned  and detailedly analyzed and the characteristics of its NMR data are also pointe d out.

The ¹H NMR ¹³C NMR spectra of 8 N-Aryl maleinamic acids  coenzyme mockup  with the productes reacted variant kind of nucleophiles were studied. The
¹H and  ¹³C chemical shifts were obtained  and their regularity has be en researched. Their elemental analysis and IR spectra have been characterized. The reaction products of eight N-aryl maleinamic acids coenzyme mockup with var ious kinds of nucleophiles were studied by ¹H and ¹³C NMR spectr oscpy.

The signal processing with Gabor wavelets is improved in this paper. When a s ignal with single frequency is selected, the interferences in both time and freq uency domains must be suppressed to minimum. Under these restraints, parameters  of the selected signals are optimized.  Moreover, it must be sure every time th at  the selected peaks are the strongest one in the difference spectrum. After all F ID with single frequency are taken out from the original FID, some huge and wide  peaks are given up for the other treatments. The remaining small peaks are reco nstructed. For liquid samples, the traditional Lorentzian function can be used g ene rally. Here, a modified Lorentzian function is given and the projections of all  sel ected signals are used for construction of a so called `projection spectrum',   or only two parameters (amplitude and frequency) are used to construct  a `lines spectrum'. It is proved by simulation and experimental data processing th at the detail information of small signals covered in a huge peak can be exposed  by means of the so called `microscope property' of  wavelets.