Abstract: In the present paper, experimental method of ³¹P NMR spectra quantitation of the phosphatic compounds in intact liver fluke Fasciola hepatica and its HClO₄ extract is described. Some preliminary quantitative results and longitudinal relaxation times T₁ of corresponding compounds are also reported. The intra-cellular pH value changes during measurement without perfusion. A per-fusion system is used to maintain Sample flukes viable. Individual peaks in ³¹P NMR spectra of HClO₄ extract are identified. Dextrose containing and dextrose depleted media incubation experiments show significant metabolic change in sugar phosphate region of spectra. By ¹³C NMR spectra, one of the major compounds α-glycerophosphoeholine in ³¹P NMR spectra is confirmed.