Abstract: ³¹P nuclear magnetic resonance (NMR) spectra of human lymphoma cell line Molt-4 were observed by using ³¹P NMR. The decrease in ATP levels and simultaneous increase in inorganic phosphate (Pi) levels occurred as the time of NMR experiment increased under temperatures of 37℃, 20℃ and 4℃.The changes in ³¹P spectra of cells slow down sequentially following the descent of NMR measurement temperature. Stable ³¹P spectrum of living cells was obtained when accumulation of data was started within 30min after the harvest of the cells and finished within 60 min at 4℃.Intracellular pH of Molt-4 cells was measured based on the chemical shift of Pi peak.

Key words: ³¹P NMR, Molt-4 cells, pH