Chinese Journal of Magnetic Resonance ›› 1996, Vol. 13 ›› Issue (5): 411-416.
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Cheng Zengjiang1, Du Zehan1, Dong Huajin2, Yan Xianzhong1, Feng Rui1
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Abstract: 31P NMR spectra of Langendorff-perfused rat and rabbit hearts were measured.Well resolved peaks of PCr, ATP, Pi, PME and GPC Were Observed over 3-5min accumulation times with enough S/N ratios to quantitate these phosphate metabolites.The energy stores of rat hearts were stable for at least 2h as shown by the good stabilities of the peak intensities of ATP and PCr,as well as the coronary flows under our experimental conditions.With the method we established,31P NMR spectra of a rat heart during 21 min ischemia and 12min reperfusion were obtained consecutively.The relative Changes between ATP, PCr and Pi Peak intensities were observed over this time course.
Key words: 31P NMR spectroscopy, Isolated hearts, High energy phosphates, Energy metabolism, Ischemia-reperfusion
Cheng Zengjiang, Du Zehan, Dong Huajin, Yan Xianzhong, Feng Rui. 31P NMR SPECTROSCOPY DETERMINATION OF LANGENDORFF-PERFUSED HEARTS[J]. Chinese Journal of Magnetic Resonance, 1996, 13(5): 411-416.
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