1H AND 13C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC)2 IN SOLUTION

Chinese Journal of Magnetic Resonance ›› 1998, Vol. 15 ›› Issue (2): 117-122.

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¹H AND ¹³C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC)₂ IN SOLUTION

Yan Jiangli^1,2, Zhang Youjie², Yuan Hanzhen¹, Mao Xi-an¹, Shen Lianfang¹

1 State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071;
2 Center for Analysis and Testing, Central China Normal University, Wuhan 430079

Received:1997-12-24 Published:1998-04-05 Online:2018-01-13

Abstract

Abstract: Deoxyoligo nucleotide d(GGTATACC)₂ was studied by two dimensional NMR techniques. Its proton and ¹³C chemical shifts were assigned by 2D proton NOE spectrum and ¹³C-¹H heteronuclear multiquantum chemical shift correlated spectrum (HMQC) combined with double quantum filtered chemical shift correlated spectrum (DQFCOSY). The sugar puckers of the eight residues of this helix octamer were determined to be dominantly C2'-endo forms with anti glycosidic conformations.

Key words: d(GGTATACC)₂, ¹H and ¹³C chemical shifts, NOESY, HMQC

Yan Jiangli, Zhang Youjie, Yuan Hanzhen, Mao Xi-an, Shen Lianfang. ¹H AND ¹³C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC)₂ IN SOLUTION[J]. Chinese Journal of Magnetic Resonance, 1998, 15(2): 117-122.

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¹H AND ¹³C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC)₂ IN SOLUTION

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