The self-diffusion coefficient, proton chemical shift and spin-lattice relaxation measurements show that the above mentioned parameters vary with concentration and showturning points which correspond to the cmc of each surfactant. The difference in variation of the chemical shifts of different resonance peaks of the surfactants provides description of the formation of micelles at a molecular level.

A new characterization method for determining the sequence distribution of ethylene-propylene copolymers, obtained with a supported Ziegler-Natta catalyst made by chemical reaction method, was carried out by ¹³C NMR spectroscopy. By dividing the ¹³C NMR spectrum into eight individual regions, triad sequence distribution and composition of EP copolymer were calculated through using fiveintegrals of these regions to avoid the use of the other three condition sensitive regions. Comparison of experimental versus calculated triad distribution points out that the first order Markovian model was more fit to the copolymer sequence distribution and the catalytic active sites are more favorable to ethylene and propylene homopoly merization.

Deoxyoligo nucleotide d(GGTATACC)₂ was studied by two dimensional NMR techniques. Its proton and ¹³C chemical shifts were assigned by 2D proton NOE spectrum and ¹³C-¹H heteronuclear multiquantum chemical shift correlated spectrum (HMQC) combined with double quantum filtered chemical shift correlated spectrum (DQFCOSY). The sugar puckers of the eight residues of this helix octamer were determined to be dominantly C2'-endo forms with anti glycosidic conformations.

A new series of polyglycol-phosphate-EDTA ester terpolymer ligands were synthesized by the copoly merization of ethylene diamine tetraacetic dianhydride(EDTAA), ethyl phosphodichloridate (or methyl phosphodichloridate, n-butyl phosphodichloridate) with diethylene glycol or triethyleneglycol, and their Fe(Ⅲ) complexes were prepared. The structures of these new ligands and Fe (Ⅲ) complexes were comfirmed by ¹H NMR, IR and elemental a nalysis. The longitudinal relaxation measurements on these macromolecular complexes indicated that most of them have higher relaxivity as compared to Fe(Ⅲ)-EDTA. Moreover, the acute toxicity test using one of these Fe (Ⅲ) complexes was performed and no obvious toxicity was observed. Finally, T₁-weighted contrast imaging of rat liver showed clear MRI enhancement after injection the new complex.

The new reaction mechanism of Pd (OAc)₂ with dppm or dppe in different chlorom ethane solvent was studied by EPR-ST method. The used capturer were PBN and POBN. The obtained experimental spectra were simulated. The free radicals·PPh₂ and containing carbon free radicals were trapped in these experiments. The reaction mechanism was given and discussed. A new ligand PPh₂CHClPPh₂ was formed after the homolysis, the deprivation of chlorine and the deprivation of hydrogen. Then the substituted and complex reaction occurred between new ligand and Pd(OAc)₂ to form a new complex PdCl₂[PPh₂CHClPPh₂]. Some influence factors on the reaction were tentatively discussed. Solvent effects play an important role on the free radical reaction because CH₂Cl₂ or CHCl₃ is both reactant and solvent. Its polarity largely acts on the transitional state 1 or 2 and determines the occurance of reaction. Pd(OAc)₂ is both reactant and initiator. It affects the reaction by its molecular induction.

Experiments were carried out using ESR spectra with 5, 5-dimethy l-1-pyrroline-Noxide (DMPO) as spin trap to study the antioxidant mechanism of glutathione (GSH), superoxide dismutase (SOD) and horseradish peroxidase (HRP). NADH-induced lipid peroxidation system was taken as an in vitro model. The results indicated that GSH could partly inhibit the generation of free radicals at low concentration, while SOD and HRP at proper ration could completely scavenge free radicals, thus prevent mitochondria lipid peroxidation from the damage of lipid peroxidation.

In this paper the EPR parameters g for Co²⁺ ions in Ⅱ-Ⅵ semimagnetic semiconductors ZnS and CdTe were studied by means of a double spin-orbit (SO) coupling model and a molecular orbit. The calculated results show that the contribution from the ligand Te to the EPR parameter g can not be neglected because the ligand Te possesses large SO coupling constant and strong covalence.

Two di-tungsten-nuclear organo-selenium compounds were studied by two dimensional NMR spectroscopy (2D ¹H, ¹³C-HMQC, HMBC and NOESY). ¹H and ¹³C chemical shifts of these two compounds are unambigously assigned.

The structure of phenylalanine transfer ribonucleic acid (tRNA^Phe) in solution was explored by ¹H nuclear magnetic resonance (¹H NMR) spectroscopy to evaluate the effect of europium ions (Eu³⁺) on the structural and conformational change. Trivalentions Eu³⁺ have specific effects on the iminoproton region of the ¹H NMR spectra of yeast tRNA^Phe. The dependence of the iminoproton spectra of yeast tRNA^Phe as a function of Eu³⁺ concentration was examined, and the results suggested that the tertiary base pair G₁₅·C₄₈, which is located on the terminal in the augmented dihydrouridine helix (D-helix) was markedly affected by Eu³⁺ (shifted downfield by 0.85). Base pair U₈·A₁₄ in yeast tRNA^Phe, which are stacked on G₁₅·C₄₈, was also affected by added Eu³⁺ when 1~2Mg²⁺ ions were also present. Ano ther imino proton that may be affected by Eu³⁺ in yeast tRNA^Phe is that of the tertiary base pair G₁₉·C₅₆. The assignment of this resonance in yeast tRNA^Phe is tentative since it is located in the region of highly overlapping resonances between 12.6 and 12.2. This base pair helps to anchor the D-loop to the TΨC loop. The binding of Eu³⁺ caused conformational change of tRNA, which is responsible for shifts to upfield or downfield in ¹H NMR spectra.

The ¹³C NMR data of 16 substituted 4-(arylamino) quinolines are reported and their structures were determined. The ¹³C chemical shifts of all 16 compounds have been assigned. The factors influencing the chemical shifts are discussed.

The solvent and temperature effects on the Keto-Enol tautomerism of 6, 8-dimethyl-1, 2, 4-triazolopyrimidine-3-butandione sulfide were studied by ¹H NMR method in chloroform, acetone, methanol and DMSO. The equilibrium constants were diminished with the rising of temperature and fairly good linear relationship were found to be present between logK and T^-1. The ΔH and ΔS in these solvents have been obtained.

NMR spectra of 6 new kinds of α,α'-dioxoketene cyclic dithioacetals are reported in this paper. The structures were determined by ¹H、¹³C NMR spectra etc., and the chemical shifts of all NMR peaks have been assigned. The effects of structures on the chemical shifts are discussed.

In this paper the calculation for ¹³C NMR chemical shift of Benzene Ring is reported in flavonoids as flavone, flavanone, flavonol, flavanonol, flavane, isoflavone and isoflavanone.

An EPR spectrum simulation software was written by using Microsoft Visual BASIC 4.0 for Windows 95. A normal calculation can be finished in less than 20s. The software was specially designed for multiple EPR simulation applications. It can be used for simulation of free radical EPR spectra, transition metal complexes/compounds EPR spectra and 2-dimensional EPR imagings. EPR imagings can be displayed in colorgrams, contour maps or 3-dimensional pictures. All the simulated images may be printed into monochrome pictures by using a HP 5L laserjet printer. By editing codes, one can choose a set of parameters for simulating calculation of different paramagnetic substances or for other data process. In the program 2560 point data are used for free radical simulation, and 128×128 for EPR imaging simulation.

The advantage and disadvantage of the method for measurement of intracellular free calcium by 19 FNMR were discussed. The application in measuring the cytosolic calcium in isolated cells, perfused hearts, isolated aorta and in vivo was reviewed.