ESR STUDY ON THE MECHANISM OF AN TI-LIPID PEROXIDATIONOF AN TIOXIDANTS

Chinese Journal of Magnetic Resonance ›› 1998, Vol. 15 ›› Issue (2): 139-144.

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ESR STUDY ON THE MECHANISM OF AN TI-LIPID PEROXIDATIONOF AN TIOXIDANTS

Gao Shujuan^1,2,3, Liu Dongbo², Luo Guimin^1,2,3, Yang Tongshu^1,2,3, Shen Jiacong³, Zhao Guangcheng⁴

1 Key Lab of Enzyme Engineering, Jilin University, Changchun, 130023;
2 Northeast Normal University, Changchun, 130024;
3 Key Lab of Supernolecular Structure and Spectrum, Jilin University, Changchun, 130023;
4 The First Teaching Hospital of Norman Bethune University of Medical Science, Changchun, 130021

Received:1997-09-01 Revised:1997-10-16 Published:1998-04-05 Online:2018-01-13

Abstract

Abstract: Experiments were carried out using ESR spectra with 5, 5-dimethy l-1-pyrroline-Noxide (DMPO) as spin trap to study the antioxidant mechanism of glutathione (GSH), superoxide dismutase (SOD) and horseradish peroxidase (HRP). NADH-induced lipid peroxidation system was taken as an in vitro model. The results indicated that GSH could partly inhibit the generation of free radicals at low concentration, while SOD and HRP at proper ration could completely scavenge free radicals, thus prevent mitochondria lipid peroxidation from the damage of lipid peroxidation.

Key words: ESR, Spin trapping, Antioxidant, Mitochondria

Gao Shujuan, Liu Dongbo, Luo Guimin, Yang Tongshu, Shen Jiacong, Zhao Guangcheng. ESR STUDY ON THE MECHANISM OF AN TI-LIPID PEROXIDATIONOF AN TIOXIDANTS[J]. Chinese Journal of Magnetic Resonance, 1998, 15(2): 139-144.

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ESR STUDY ON THE MECHANISM OF AN TI-LIPID PEROXIDATIONOF AN TIOXIDANTS

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