Chinese Journal of Magnetic Resonance ›› 2010, Vol. 27 ›› Issue (1): 22-31.

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Studies of Antioxidation Activity of Natural Products with EPR Methods

  

  1. State Key Laboratory of Natural and Biomimetic drugs, Health Science Center of Peking University, Beijing 100191, China
  • Received:2009-12-30 Published:2010-03-05 Online:2010-03-05
  • Supported by:

    Unilever Cooperation Research, National 9.5 Climb Plan Grant.

Abstract:

Many of EPR(electron paramagnetic resonance) experimental methods can be used in studies of antioxidation activities of natural products, such as spin trapping EPR, cryogenic EPR for modeled animals and spin labeling EPR et al. It is an elementary requirement for an antioxidant to scavenge oxygen free radicals in vitro, but evaluating method in vivo is more valuable when the antioxidant is applied to a biological system. Through the studies of more than 200 herbages and some TCM recipes, the EPR screening methods for natural products as antioxidants have been established and described in detail. With the above EPR methods, it is believed that antioxidation activities of natural products can be obtained at molecular, cell, and tissue levels, respectively.

Key words: EPR, Oxygen Free Radical, Antioxidation, Natural Product

CLC Number: