Abstract: In this paper, the exchange rates of slowly exchanging amide protons in human insulin R6 hexamer were obtained by using NMR Linear Prediction Model Method. Because the method is able to combine information from independent prediction calculation and to exploit differences in chemical shift and exchange rate simultaneously, it allows exchange rates of amide protons with closely spaced signal to be determined. The values of exchange rates from calculation are in good agreement with those from 2D NMR spectrum. The relationship between exchange rates and structure of the protein are discussed in the paper.

Key words: NMR, Exchange, Amide protons