31P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4

Chinese Journal of Magnetic Resonance ›› 1996, Vol. 13 ›› Issue (2): 107-112.

³¹P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4

Huang Rongqing¹, Du Zehan², Yang Zhigang³, Feng Rui²

1. Institute of Radiation Medicine;
2. National Centre of Biomedical Analysis;
3. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850

Received:1995-07-03 Revised:1995-08-07 Published:1996-04-05 Online:2018-01-17

Abstract

Abstract: ³¹P nuclear magnetic resonance (NMR) spectra of human lymphoma cell line Molt-4 were observed by using ³¹P NMR. The decrease in ATP levels and simultaneous increase in inorganic phosphate (Pi) levels occurred as the time of NMR experiment increased under temperatures of 37℃, 20℃ and 4℃.The changes in ³¹P spectra of cells slow down sequentially following the descent of NMR measurement temperature. Stable ³¹P spectrum of living cells was obtained when accumulation of data was started within 30min after the harvest of the cells and finished within 60 min at 4℃.Intracellular pH of Molt-4 cells was measured based on the chemical shift of Pi peak.

Key words: ³¹P NMR, Molt-4 cells, pH

Huang Rongqing, Du Zehan, Yang Zhigang, Feng Rui. ³¹P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4[J]. Chinese Journal of Magnetic Resonance, 1996, 13(2): 107-112.

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³¹P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4

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