Abstract: The nonsymmetric 2D NOESY method is proposed to measure the full 2D NOESY mixing coefficient matrix. Theoretical researchs showed that the nonsymmetric 2D NOESY has a nonsymmetric peak intensity matrix and a higher signal-to-noise ratio than the conventional one. Therefore, it is possible that the full 2D NOESY mixing coefficient matrix can be obtained only from the off-diagonal elements of peak intensity matrix of the nonsymmetric 2D NOESY, which is impossible for the conventional 2D NOESY, and the peak intensity measurement of overlapping diagonal elements of the nonsymmetric 2D NOESY in such spin systems as biomacromolecules may be avoided doing. In the final the theoretical results were confirmed with numerical simulated studies.

Key words: Nonsymmetric 2D NOESY, 2D NOESY mixing coefficient matrix, Nuclear cross-relaxation rate, Internuclear distance