QUALITATIVE AND QUANTITIVE DETERMINATION OF STEROIDS IN MICRO-QUANTITY SAMPLES USING 1H NMR SPECTROSCOPY

Chinese Journal of Magnetic Resonance

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QUALITATIVE AND QUANTITIVE DETERMINATION OF STEROIDS IN MICRO-QUANTITY SAMPLES USING ¹H NMR SPECTROSCOPY

XUE Song, HU Jie-han, ZHANG Wei*, YU Xing-ju, YUAN Quan

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China

Received:2002-12-30 Revised:2003-02-21 Published:2003-06-05 Online:2003-06-05
About author:*Correspondence author：ZHANG Wei.
Supported by:
国家海洋863项目(2001AA624040).

Abstract

Abstract:

5β-CHOLESTAN-3β-OL was determined as the major steroids in marine sponge -Hymeniacidon perleve- with a level of ca. 1 000 ppm. Cholesterol was fou
nd in the 5β-CHOLESTAN-3β-OL crystal using ¹H NMR spectroscopy, while GC-MS failed to obtain the same results. Using ¹H NMR spectroscopy, standard mixtures of 5β-CHOLESTAN-3β-OL and cholesterol were studied to accurately quantify the steroids that are difficult to be separated by chromatography.

Key words: NMR, quantity, steroid, marine sponge, Chromatography, Hymeniacidon perleve

CLC Number:

O482.53

XUE Song, HU Jie-han, ZHANG Wei*, YU Xing-ju, YUAN Quan. QUALITATIVE AND QUANTITIVE DETERMINATION OF STEROIDS IN MICRO-QUANTITY SAMPLES USING ¹H NMR SPECTROSCOPY[J]. Chinese Journal of Magnetic Resonance.

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QUALITATIVE AND QUANTITIVE DETERMINATION OF STEROIDS IN MICRO-QUANTITY SAMPLES USING ¹H NMR SPECTROSCOPY

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