Abstract: A diosgenyl saponin has been extracted from the rhizomes of Paris Polyphylla Smith, and determined to be diosgenin 3-O-α-L-rhamnopyranosyl-(1→2)[α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside. The NMR chemical shifts of the compound have been assigned using 1D and 2D NMR spectroscopy, including ¹H-¹H COSY, HSQC and HMBC. Selective 1D TOCSY experiments have been performed to assign the crowded spectral region from glycosidic protons.

Key words: NMR, 2D NMR, 1D TOCSY, glucopyranoside