Abstract: Ploy-substituted benzene can be simply synthesized via acyclic precursor through intramolecular witting reaction.But the relative situation between each o f the substituents can not be easily confirmed by the empirical calculation of chemical shift of aromatic carbon through normal 1D NMR experiments.
2D COLOC NMR technique is an efficient method to determine the location of each substituent of poly-substituted benzene,This paper deals with the application of COLOC to the structural identification.The difference between the calculated and the measured chemical shift of each aromatic carbon and the ³J_CF coupling constants were discussed.

Key words: NMR, COLOR, Ploysubstituted benzene, Substituent