NMR STUDIES Of HARRINGTONINE INDUCED APOPTOSIS IN HL-60 CELLS

Abstract

Abstract:

NMR spectroscopy methods were used to study the metabolic changes of A TP, PME and lipid during harringtonine (HT) induced apoptosis in HL-60 cell. After HL-60 cells were treated with HT for two hours, it was observed by ³¹P-NMR sp ectroscopy that the levels of ATP and PME decreased significantly and intracellu lar pH increased significantly. It was also found by ¹H-NMR a significa nt increa se in the signal intensity of methylene resonance and a slight decrease in choli ne peak in treated HL-60 cells. The present investigation will be helpful for understanding the mechanism of Harringtonine induced apoptosis in HL-60 cells.

Key words: NMR, spectroscopy, HL-60, harringtonine (HT)

CLC Number:

O482.53

WU Chang-lin1, QIAN Min2, HUANG Yong-ren1. NMR STUDIES Of HARRINGTONINE INDUCED APOPTOSIS IN HL-60 CELLS[J]. Chinese Journal of Magnetic Resonance.

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