AN OUTLINE OF NMR STUDIES OF DNA

Abstract

Abstract:

Applications of NMR to the studies of DNA structures and dynamics are reviewed in aspects regarding NMR peak assignment, NMR constraints and structure calculation, main features of DNA NMR spectra, ring current effect on DNA chemical shifts, and DNA dynamics in solution. Unsolved problems in DNA NMR studies are also pointed out, which include: (1) The dihedral angle parameters in molecular modeling force field are not consistent with those in irregular DNA. (2) DNA structures simulated by molecular modeling usually do not have good consistency. (3) The ring current theory for explaining DNA chemical shifts is yet not ready for use. (4) The “modelfree” model has difficulties in interpreting steady-state NOE. Nonetheless, the structural and dynamical information provided by NMR is still important, and has greatly deepened our understanding of DNA.

Key words: NMR, DNA, Outline, Questions remaining unsolved

CLC Number:

O482.53

MAO Xi-an. AN OUTLINE OF NMR STUDIES OF DNA[J]. Chinese Journal of Magnetic Resonance.

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