Abstract: A series of 1D (¹H and ¹³C NMR, DEPT experiment) and 2D NMR (including gCOSY, gHSQC and gHMBC) techniques were applied to the assignment of all proton and carbon signals of forbesoside [6′-O-(trans-feruloyl)-nodakenin] which was a new coumarins compound isolated from the underground parts of Notopterygium forbesii Boiss. and Notopterygium incisum Ting ex H. T. Chang and with anti-inflammatory bioactivity. The NMR signals changed rules from umbelliferone to 7-demethylsuberosin, nodakenetin, nodakenin and forbesoside, and of acyled hydroxymethyl moiety of glucopyranosyl group, and methods of NMR signals assignment of saccharide were discussed and summarized.

Key words: NMR, forbesoside, 6′-O-(trans-feruloyl)-nodakenin, umbelliferone, nodakenetin, Notopterygium forbesii and Notopterygium incisum