Abstract: The low intrinsic sensitivity of 2D heteronuclear NOE (HOESY) experiment has hampered its applications in structural analysis. The sensitivity of the HOESY experiment can be improved by ¹H detection (inversed detection). Here we present a 2D ¹³C-¹H HOESY experiment with presaturation to further improve its sensitivity by NOE enhancement. In the proposed experiment, t₁ noises in the ¹H detected HOESY spectra are suppressed by pulse field gradient (PFG) coherence selection and phase cycles. It was demonstrated that ¹³C-¹H NOE correlation spectra with improved sensitivity could be obtained with the proposed experiment.

Key words: NMR, heteronuclear NOESY, presaturation, adenosine, sensitivity improvement