Cathepsin B Triggered Hyperpolarization 129Xe MRI Probe for Ultra-Sensitive Lung Cancer Cells Detection

doi:10.11938/cjmr20202828

Abstract

Abstract:

Cathepsin B (Cat B) is a lysosomal cysteine protease that plays an essential role in cellular metabolism. Cat B is overexpressed in lung cancer cells. Fluorescence imaging techniques have been developed for measuring intracellular Cat B levels, which, however, suffers from limited penetration depth and interferences from autofluorescence background. To resolve these technical difficulties, we designed a novel Cat B biosensor detected by hyperpolarized xenon magnetic resonance imaging. It consists of a cryptophane cage as a ¹²⁹Xe NMR reporter and an amide bond as a Cat B-specific cleavable group. When the biosensor interacts with Cat B, the cleavage of amide bond leads to changes in ¹²⁹Xe chemical shift. Combing with hyperpolarization-chemical exchange saturation transfer (Hyper-CEST), our biosensor provides a novel method for responsive detection of Cat B.

Key words: nuclear magnetic resonance (NMR), contrast agents, lung cancer, hyperpolarization ¹²⁹Xe MRI probe, cathepsin B, chemical exchange saturation transfer (CEST)

CLC Number:

O482.53

Chong-wu WANG,Xi HUANG,Lei SHI,Shi-zhen CHEN,Xin ZHOU. Cathepsin B Triggered Hyperpolarization ¹²⁹Xe MRI Probe for Ultra-Sensitive Lung Cancer Cells Detection[J]. Chinese Journal of Magnetic Resonance, 2021, 38(3): 336-344.

Figures/Tables 9

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References 34

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Cathepsin B Triggered Hyperpolarization ¹²⁹Xe MRI Probe for Ultra-Sensitive Lung Cancer Cells Detection

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