Chinese Journal of Magnetic Resonance ›› 2026, Vol. 43 ›› Issue (2): 175-185.doi: 10.11938/cjmr20253177cstr: 32225.14.cjmr20253177
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CAI Yue1,2,*(
), WANG Xuxia1,2, LIU Sijie1,2, GUO Haodong1,2, CHEN Xi1,2, CHENG Linlin1,3, KANG Yan1,2, LIN Fuchun1,2
Received:2025-07-23
Published:2026-06-05
Online:2025-09-08
Contact:
CAI Yue
E-mail:caiyue192@mails.ucas.ac.cn
CLC Number:
CAI Yue, WANG Xuxia, LIU Sijie, GUO Haodong, CHEN Xi, CHENG Linlin, KANG Yan, LIN Fuchun. Magnetic Resonance Imaging Study on the Microstructure Abnormalities of Striatal White Matter in Type 2 Diabetic Mellitus Rats[J]. Chinese Journal of Magnetic Resonance, 2026, 43(2): 175-185.
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Table 1
Experiment reagents and chemicals
| 试剂名称 | 纯度 | 供应商 |
|---|---|---|
| 链脲佐菌素（STZ） | ≥99% | S0130, Sigma |
| 戊巴比妥钠 | ≥99% | 57-33-0, Sigma |
| 柠檬酸-柠檬酸钠缓冲液 | 0.1 mol/L, pH=4.5 | pH1716，飞净 |
| 0.9%生理盐水 | 0.9% (w/v) | 武汉滨湖双鹤药业有限公司 |
| 丙三醇 | ≥99% | 国药集团化学试剂有限公司 |
| 乙二醇 | ≥99.5% | 国药集团化学试剂有限公司 |
| 多聚甲醛 | ≥95% | 国药集团化学试剂有限公司 |
| 氯化钠 | ≥99.5% | 国药集团化学试剂有限公司 |
| 氯化钾 | ≥99.5% | 国药集团化学试剂有限公司 |
| 十二水合磷酸氢二钾 | ≥99% | 国药集团化学试剂有限公司 |
| 磷酸二氢钾 | ≥99.5% | 国药集团化学试剂有限公司 |
| 氢氧化钠 | ≥99.8% | 国药集团化学试剂有限公司 |
| 羊血清 | ≥99% | 武汉飞弈科技有限公司 |
| 异氟烷 | ≥99% | 深圳市瑞沃德生命科技股份有限公司 |
| 葡萄糖 | ≥99.5% | 国药集团化学试剂有限公司 |
| 蔗糖 | ≥99% | 国药集团化学试剂有限公司 |
| 髓鞘碱性蛋白(MBP)一抗 | ≥95% | Ab218011, sigma |
| 磷酸化神经丝(SMI-31)一抗 | 纯化型 | SMI-31P-100, Covance |
| 二抗羊抗小鼠 Alexa Fluor 594 | 免疫原亲和纯化型 | ab150116, abcam |
| 二抗羊抗兔 Alexa Fluor 488 | 免疫原亲和纯化型 | ab150077, abcam |
Fig. 1
The pipeline of whole experiment. CON: the saline control group rats; T2DM: the T2DM group rats; NCD: normal chow diet; HFD: high-fat diet; STZ: streptozotocin; OGTT; oral glucose tolerance test
Fig. 2
The changes in body weight and fasting blood glucose level in rats. (a) Changes in body weight of rats in the two groups from 4 to 16 weeks of age; (b) Changes in fasting blood glucose in the two groups of rats aged 4 to 16 weeks. *p < 0.05; ***p < 0.001
Fig. 3
Oral glucose tolerance test in rats. (a) The dynamic change curves of blood glucose in the OGTT test of the two groups of rats; (b) The areas under the OGTT change curves of the two groups of rats, with ***p < 0.001
Fig. 4
Effect of T2DM on the microstructure of white matter in rats. CPU: caudate putamen (striatum), M1: primary motor cortex, S1FL: primary somatosensory cortex, forelimb area, S1HL: primary somatosensory cortex, hindlimb area. p<0.005 (FDR correction), voxel clusters of RARE =100, voxel clusters of DTI =50
Fig. 5
Immunohistological staining of the striatum in rats. (a) Immunohistochemical staining of MBP and SMI-31 in the striatum region of the two groups of rats. The scale of MBP immunoresponse staining was 200 μm, and the scale of SMI-31 immunoresponse staining was 20 μm; (b) The IOD value of MBP in the striatum region; (c) The IOD value of SMI-31 in the striatum region. * p < 0.05; ** p < 0.01
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