Abstract: The principle of pulse sequences of improved ROESY (CAMELSPIN) to suppress the off-resonance effects and increase the sensitivity of spectra were introduced. The 1D and 2D ROESY experiments were performed. The results of experiments show that the enhancement of ROE signals in the spectra of peptides and proteins can be obtained up to about 40% or 80% compared with the conventional ROESY experiment, and almost other 2D NMR resonances were vanished from the ROESY spectrum.

Key words: ROESY, Off-resonance effect, NMR