¹H spin system identifications of amino acid residues are the bases of investigation of structrue, function and motion of proteins by use of NMR techniques. 2D DQ-spectrum is an important method for the identification. 500 MHz and 300 MHz phase sensitive 2D DQ-spectra of Des-(B₂₆-B₃₀)-insulin in H₂O and S-sulfonated insulin A chain in D₂O were measured respectively. From their direct, remote and equivalence cross-peaks, the spin system identifications of various residues were carried out which confirmed the identifications from the COSY spectra and provided more information for the identification.

The ¹¹⁹Sn,¹¹⁷Sn-NMR spectra were recorded for twenty organo-tin (IV) compounds containing alkyl, phenyl, allyl, five-membered heterocyclcs and other polar groups. The substituent influence on tin electron density and magnetic shielding were discussed. The effects of solvents, concentration and temperature on ¹¹⁹Sn resonances were investigated. The complex formation of Ph₃SnCl with DMSO was studied and the equilibrium constant K and △G were calculated.

The tracer experiments of the passing CO-HCl mixture through Toluene-Aluminum Chloride-Hydrogen Chloride system were described by ¹³C-NMR.The results suggest that the intermidiate complex of thecarbonylation of Toluene is  AlCl₄^-, the reaction mechanism is also discussed.

In this paper, a new method was suggested to separate Dipole-Dipole relaxation, that is called parameter separation method. For a stable rigid molecule, the diffusion coefficient could be calculated from measurement values of secondry and tertiary carbons, which is usually not only for a kinematic research, but also for evaluation of Dipole-Dipole relaxation of quaternary carbons. This procedure shows higher reliability. The result of relaxation study on title mentioned compound shows that parameter separation method is quite efficient for the study of relaxation mechanism for a stable rigid molecule, especially for quaternary carbons.

The correlation functions of side-groups of polymers were given for NMR relaxation if the polymer chains undergo scgmental motion, these functions were demved from nonequivalent two sites and three sites jump internal rotations, stochastic diffusion internal rotation and restricted internal rotation. The corresponding spectral density functions were also given, and two examples were discussed.

A stetic capillary mcthcd of ESR has been used to investigate the chemical reactions of gentisic acid in different alkaline solutions. The ESR spectra of chemical reactions of gentisic acid in sodium alkoxide-alcohol were different from those in sodium hydroxide aqueous solution. The difference was given as follows:
1. The chemical reactions of gentisic acid in sodium alkoxide-alcohol solution were autcoxidation. Three new free radicals in the chemical reactions were found and their structural models were given.
2. The chemical rearlions of gentistic acid in sodium hydroxide aqueous solution were first autooxidation and then substitution. A new free radical in the autooxidation step was found and its structural model was given^[1]. In the substicittiea step the hydrogen(H) on the benzene ring of the gentisic acid was substisated by the hydroxyl group (OH) in the solution.

The photolysis of 2, 1, 3-benzoxadiazole-1-oxide in the presence of various organic amines have been investigated using ESR spectroscopy. Our results show that nitroxide radicals are the stable products of the photooxidation of the corresponding organic amines. Oxygen-transfer exciplex derived from the triplet state of 2,1,3-benzoxadiazole-1-oxide is an intermediate to give nitroxide through N-H bond cleavage in the organic amine within the exciplex.

ESR measurements have been performed on polyacenic material prepared by the pyrolytic treatment of phenol-formaldehyde resin. Five pyrolysis temperatures in the range of 500-1100℃ were selected. The ESR line width, spin concentration, the ratio of Dysonian lineshape's A and B etc. of the pristine samples strongly depend on the pyrolysis temperatures, reflecting the structureal characteristics in each samples.

Samples of pine tree ring from Mt. Tuomuer region of Xinjiang province were researched using ESR method. The result shows that the free radical concentration N_g is an index of tree growth and relates to the season, and to the temperature and the precipitation. The N_g of spring wood is more than that of autumn. The N_g of wood was prominently influenced by the period of sunspot activity and it varies with the solar period, but the values of peak and valley have been preacted 2-3 years or postponed.

This is a method for the determination of the fraction of end hydroxyl by measuring the ester-H ratio of ¹H-NMR absorption peak areas for the partly esterified polyether and the completely esterified polyether. The molecular weight of polyether can be determined by ester-H groups.

The ¹H NMR spectra of N-methyl-isoindigatin in(CD₃)₂CO,CDCl₃, DMSO-d₆ and after D₂O exchange were investigated by means of selective irradiation method. The ¹H-¹H coupling constants and the assignment of ¹H NMR spectral lines of protons with extremely similar chemical envelopments were obtained.

The ESR zero field signal observed at and under T_C temperature in copper oxide superconductor is assigned to be a configuration transition of the copper oxide complex between the degenerate state E and the single state A discussed by R. Englman and others^[1-3].

Molecular structure of the δ-Damascones were defined by ¹³C-NMR technique (BB, DEPT, Gated decoupling). The influence space configuration and shielding effect on δ_C was discussed, and primary characteristic parameters distinguishing isomer(Ⅰ) from(Ⅱ) have been obtained.

¹H and ¹³C NMR spectra of N (,N')-(di) tosyl macro-cyclic ether-bislactones (1-6), N,N'-dicarboxymethyl macrocyclic ether-bislactones and amine-bislactame (8-12) and 4'-dansylamino benzo crown ethers (13-16) etc. eighteen crown ethers were recorded and assigned by consideration of known substitute effects. The ¹H chemical shifts obtained indicate that the larger the crown ether, the larger the proton chemical shift for methylene b in Crown 1-6 and for methylene a, b, c in Crown 8-11. These shifts approach a limit when the number of macrocyclic atoms arrives at 21-24. Solvents show considerable effect on the ¹H chemical shifts of methylenes a, b, c in Crown 8-11 which increase with the solvent polarity, the order is as follows:CD₃COOD > PY-d₃ ≥ D₂O > CD₃OD > CD₃CD₂OD > DMF-d₇ > DMSO-d₆.

¹H NMR data of compounds I and Ⅱ reveal that both compounds are diamagnetic and stable in dimethylsulfoxide solution as their molecular structures remain the same as those in the solid state. NMR parameters are estimated by comparing the simulated spectra with the observed ones. Diamagnetic properties are confirmed by ¹³C NMR measurement.

The principle of pulse sequences of improved ROESY (CAMELSPIN) to suppress the off-resonance effects and increase the sensitivity of spectra were introduced. The 1D and 2D ROESY experiments were performed. The results of experiments show that the enhancement of ROE signals in the spectra of peptides and proteins can be obtained up to about 40% or 80% compared with the conventional ROESY experiment, and almost other 2D NMR resonances were vanished from the ROESY spectrum.

Principles and computer flowchart of N-SIMPLEX are introduced. An example is provided to illustrate the applications for obtaining NMR parameters.

The present way of microwave power adjustment of Bruker ESR spectrometer (ER-200 D; ESP-300) is digital. It provides a skipping power output by a DB appliance. According to its controlling principle, we realized to improve the way of microwave power adjustment to a continuous one without much more changing its original circuit. It is practicable and useful proved with some tests.