Abstract:

We report a methodology that allows identification of natural mixtures, using difference spectra of several NMR spectra. During the study on natural products from marine sponge Hymeniacidon perleve we obtained the consecutive elutates by column chromatography with the eluent(the value of ε₀ from 0.2～0.3) then NMR and GC-MS were used to determine the composition of the elutates. By comparing the spectra of the elutates we could classify the peaks into several groups and assign the partial peaks to identify the compounds. In the process we obtained the relationship between elutates and ε₀. 

Key words: NMR,  natural product mixtures, phthalates, marine sponge, Hymeniacidon perleve