Chinese Journal of Magnetic Resonance ›› 2010, Vol. 27 ›› Issue (1): 89-94.

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A Cell Perfusion System for Online NMR Studies

  

  1. 1.Chemistry Department, Guizhou Normal University, Guiyang 550001, China;
    2.Chemistry Department, Guizhou Bijie College, Bijie 551700, China;
    3.Center for R & D of Fine Chemicals, Guizhou University, Guiyang 550025, China
  • Received:2009-12-30 Published:2010-03-05 Online:2010-03-05
  • Supported by:

    国家自然科学基金资助项目(20765001);贵州省科技基金资助项目[黔科合J字(2006)2012].

Abstract:

A cell perfusion system for online NMR studies is reported. The system was used to measure 31P NMR signals of ATP in living breast cancer cells (MCF-7), with or without perfusion with cantharidin solution. The results- showed that online NMR on perfused cell culture is an effective way to identify active components from extracts of traditional Chinese medicine. The method can also be used to study the interactions between drugs and cancer cells.

Key words: NMR, cell perfusion, 31P NMR

CLC Number: